Fig. 5: ATF4 promotes mycolactone-mediated cytotoxicity. | Cell Death & Disease

Fig. 5: ATF4 promotes mycolactone-mediated cytotoxicity.

From: Inhibition of Sec61-dependent translocation by mycolactone uncouples the integrated stress response from ER stress, driving cytotoxicity via translational activation of ATF4

Fig. 5

a, b Wild-type HeLa cells and two different ATF4−/− clones (3 and 5.5) were treated with either DMSO, mycolactone (MYC) or starved of leucine (Leu−). a Lysates from 24-h-treated cells were analysed by immunoblotting. b After 4 days the % survival of cells was determined by staining of cells with propidium iodide (PI), cell event (detects active caspase 3/7) and DRAQ5. The number of live cells (negative for both active caspase 3/7 and PI) in three fields was determined and expressed as a proportion of total cells (Mean ± SEM, n = 3 independent experiments). c−e HeLa cells were treated as shown or with LY294002 (LY) for 1 h. Equal protein quantities in lysates were analysed by immunoblotting. All immunoblots show the approximate migration of molecular weight markers in kDa. See also Fig. S5

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