Fig. 2: The gene expression profile demonstrates the broad and multiple effects of HDAC3 on gene expression after PH. | Cell Death & Disease

Fig. 2: The gene expression profile demonstrates the broad and multiple effects of HDAC3 on gene expression after PH.

From: Histone deacetylase 3 promotes liver regeneration and liver cancer cells proliferation through signal transducer and activator of transcription 3 signaling pathway

Fig. 2

a A heat map of 1557 differentially expressed genes (fold changes ≥1.5 and p < 0.05) in the control and mutant livers at 36 h after PH compared with their corresponding baseline values (0 h). b mRNA microarray analysis of cell cycle-related genes. Differentially expressed genes (fold changes ≥1.25 and p < 0.05) in the control and HDAC3△ livers at 36 h after PH. c GO term analysis of the list of differentially expressed genes (fold changes ≥1.5 and p < 0.05) indicated that the IL6/STAT3 pathway is dramatically changed during liver regeneration in the HDAC3△ mice after PH. d Heat map of the selected genes from the microarray analysis of the control and HDAC3△ livers. e Quantitative RT-PCR confirmation of the downregulated early-response genes in the HDAC3△ livers vs. the control liver at 36 h after PH. f qRT-PCR confirms downregulation of cyclin D1 and c-myc in the HDAC3△ livers vs. the control liver at 36 h after PH. All data represent the mean ± SD; n = 3; **p < 0.01; ***p < 0.001; n.s. not significant

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