Fig. 5: PFKFB3 inhibitor PFK15 inhibits HCC growth in vivo and in vitro.
From: PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT
a CCK8 assay for cell proliferation of SMMC7721 cells and Huh7 cells treated with PFK15 in a dose and time series. PFK15 inhibited proliferation of SMMC7721 and Huh7 in a dose-dependent and time-dependent manner. b Flow cell apoptosis detection of the cell apoptosis rate of SMMC7721 cells and Huh7 cells treated with PFK15 in a dose and time series. PFK15 increased the apoptosis rate of SMMC7721 and Huh7 in a dose-dependent and time-dependent manner. c Flow cytometry cycle detection of the cell cycle ratio of SMMC7721 cells and Huh7 cells treated with PFK15 in a dose and time series. PFK15 induced G2/M arrest of SMMC7721 and Huh7 in a dose-dependent and time-dependent manner. d Comet assay for DNA damage of SMMC7721 cells and Huh7 cells treated with PFK15 (24 h). PFK15 led to DNA damage in vitro. e Comparison of tumor sizes of the SMMC7721-control group and SMMC7721-PFK15 group (1062.2 ± 578.2 mm3 vs. 215.9 ± 104.8 mm3; p = 0.03), and comparison of tumor sizes of the Huh7-control group and Huh7-PFK15 group (1628.4 ± 495.5 mm3 vs. 475.4 ± 222.9 mm3; p = 0.004). PFK15 treatment delayed tumor growth in both SMMC7721 and Huh7 tumor models. f Representative immunohistochemistry of liver cancer showing the expressions of Ki67 and ERCC1 from Balb/c nu/nu mice orthotopically implanted with SMMC7721 or Huh7 cells. PFK15 treatment decreased the expression of ERCC1 and Ki67.(Magnification ×200). g Representative TUNEL fluorescence of liver cancer from Balb/c nu/nu mice orthotopically implanted with SMMC7721 or Huh7 cells. PFK15 treatment increased the apoptosis rate of tumor.(Magnification ×200). **p < 0.01
