Fig. 3: Kir6.1 promoted M2 microglia polarization.
From: Kir6.1/K-ATP channel modulates microglia phenotypes: implication in Parkinson’s disease
a–g Kir6.1 knockdown reduced the expression of related M2 markers. The expression of Arginase1 (a), CD206 (b), YM-1 (c), IL-10 (d) and TGF-β (e) were assessed by qPCR or ELISA in microglia. f Representative immunofluorescence staining of CD206 was visualized under microscopy. g The expression of M2 related biomarker MGL1/2 was detected by flow cytometry. Data are presented as mean ± SEM from four independent experiments, ***p < 0.001 versus corresponding control; #p < 0.05, ##p < 0.01 versus IL-4-treated negative control (NC) groups. h–k Kir6.1 overexpression increased the expression of related M2 markers. The expression of Arginase1 (h) and YM-1 (i) were assessed by qPCR. j Representative immunofluorescence staining of CD206 was visualized under microscopy. k The expression of M2 related biomarker MGL1/2 was detected by flow cytometry. Data are presented as mean ± SEM from four independent experiments, **p < 0.01, ***p < 0.001 versus corresponding control; ##p < 0.01 versus IL-4-treated vector groups
