Fig. 2: Knockdown of LASP1 in NPC cell inhibits cell proliferation, invasion, and metastasis, and enhances cell apoptosis. | Cell Death & Disease

Fig. 2: Knockdown of LASP1 in NPC cell inhibits cell proliferation, invasion, and metastasis, and enhances cell apoptosis.

From: LASP1 promotes nasopharyngeal carcinoma progression through negatively regulation of the tumor suppressor PTEN

Fig. 2

a Western blot and real-time PCR were performed to detect the protein and mRNA expression of LASP1 in 5-8F cells transfected with sh-LASP1 and sh-NC. The numbers under the band represent the relative immunosignal values of the protein. b Cell growth evaluated by the CCK-8 assay (left) and representative images of colony formation assay plates (right). The number of colonies containing >50 cells were counted. The error bars represent mean ± SD from three independent experiments. c The cell cycle phases of treated cells were evaluated by flow cytometry after being transfected with sh-LASP1 and sh-NC. The data are expressed as means ± SD (n = 3). d Flow cytometric analysis of apoptosis in vitro after treatment of cisplatin (100 μM) for 12 h. e Invasion and migration in 5-8F cells decreased after being transfected with sh-LASP1 in the transwell assay. Results are expressed as mean ± SD of three independent experiments. f Wound healing was delayed in shRNA-transduced cells compared to sh-NC cells after 48 h. Results are expressed as mean ± SD of migrated distance from three different points. Migrated distance = width (0 h) – width (48 h)

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