Fig. 3: miR-30a-5p represses GBC cell cycle progression and EMT in vitro.

a Representative results of the cell cycle analysis in which specific cell lines were treated with miR-NC and miR-30a-5p. b Subcellular β-catenin expression in indicated cells was detected by western blotting. β-actin and Histone H1 were used as a loading control. c E-cadherin and vimentin protein expression levels in the indicated cells were examined by western blotting. d E-cadherin and vimentin protein expression levels in the indicated cells were examined by Immunofluorescence. The nuclei were counterstained with DAPI. Scale bar = 100 μm