Fig. 4: Ube2s promoted mES cell differentiation into mesoendoderm. | Cell Death & Disease

Fig. 4: Ube2s promoted mES cell differentiation into mesoendoderm.

From: Ube2s stabilizes β-Catenin through K11-linked polyubiquitination to promote mesendoderm specification and colorectal cancer development

Fig. 4

a, b Ube2s overexpression stable mES cell line U1 and the vector-transfected control line (HA) were induced to differentiate into mesoendoderm. The resulting cells were analyzed by qRT-PCR (a; t-test, **p < 0.01) and immunocytochemistry staining assay with antibodies against HA (Ube2s), T and DAPI (b). Undifferentiated mES cells (wild-type, WT) were used as controls. Photographs were taken with 200× magnification. c The mean DAPI-normalized staining signals of T from b were quantified by NIH Image J software. The values represent ratios against WT (lower panel; t-test; ***p < 0.001). All data presented are based on three independent repeats

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