Fig. 6: UBE2S enhanced the ability of colorectal cancer cells in migration and invasion in vitro and in vivo. | Cell Death & Disease

Fig. 6: UBE2S enhanced the ability of colorectal cancer cells in migration and invasion in vitro and in vivo.

From: Ube2s stabilizes β-Catenin through K11-linked polyubiquitination to promote mesendoderm specification and colorectal cancer development

Fig. 6

a The scratch assay monitored the migration capacity with the HCT116 cells expressing exogenous UBE2S and β-CATENIN shRNAs. The dox-untreated and dox-treated HCT116 cells without β-CATENIN depletion were used as controls. b The scratch assay examined the effect of UBE2S depletion on HCT116 cell migration. c The cells of a were examined by the transwell assay. Five fields were randomly picked to count the cells which invade the lower surface of the filter for statistical analysis. d The transwell assay examined the effect of UBE2S depletion on HCT116 cell invasiveness. Similar statistical analysis with c. e UBE2S promotes lung metastasis of CRC cells. The left panel: representative images of lungs isolated from the nude mice. The paraffin-embedded lung tissues were analyzed by H&E staining; the right panel: the number of metastasis colonies (≥1 mm in diameter) per lung were counted; t-test: ***p < 0.001; **p < 0.01; *p < 0.05. The data presented in a–d are based on three independent repeats. The number of mice for each group in e is seven

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