Fig. 7: GADD45α inhibits APE1 cytoplasmic localization via suppressing NO production in cervical cancer cells.
From: GADD45α sensitizes cervical cancer cells to radiotherapy via increasing cytoplasmic APE1 level
a Levels of NO, eNOS, and iNOS were significantly increased in HeLa-XR cells compared to HeLa cells. Cellular levels of NO, eNOS, and iNOS were measured using an ELISA Kit. b Silencing GADD45α significantly increased radiation-induced NO, eNOS, and iNOS in HeLa cells. c Overexpression of GADD45α significantly inhibited radiation-induced NO, eNOS, and iNOS in HeLa-XR cells. d NOS inhibitor treatment prevented GADD45α depletion-induced cytoplasmic APE1 accumulation in HeLa cells. Subcellular APE1 localization was detected using immunohistofluorescence. HeLa cells that were transfected with GADD45α shRNA or vector were treated with NOS inhibitor. After 12 h inhibitor treatment, immunofluorescence (IF) assays were performed. Each experiment was repeated three times. Red: APE1; blue: DAPI. *p < 0.05; **p < 0.01; ***p < 0.001
