Fig. 5: GTP-Rac1 and pAKT did not participate in TCF/β-catenin signaling pathway after SLP-2 inhibition in NSCLC cells. | Cell Death & Disease

Fig. 5: GTP-Rac1 and pAKT did not participate in TCF/β-catenin signaling pathway after SLP-2 inhibition in NSCLC cells.

From: Stomatin-like protein 2 regulates survivin expression in non-small cell lung cancer cells through β-catenin signaling pathway

Fig. 5

a Western blot analyses for the expressions of pAkt in A549, H460, H838, and H157 cells treated with either AdSLP2i or AdCtrl at m.o.i. of 100. b Downregulation of SLP-2 increased the GTP-Rac1 proteins in A549 and H157 cells, but decreased the GTP-Rac1 proteins in H838 and H460 cells as determined by pull-down assay. Total cell lysates were subjected to the pull-down assay for GTP-Rac1 activities. Total cell lysates were analyzed for Rac1 expression as a loading control. The ratio of GTP-Rac1/total Rac1 in cells was analyzed by densitometry of the blot

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