Fig. 2: M2-polarized macrophages induce aggressive behavior of human and mouse GC cells. | Cell Death & Disease

Fig. 2: M2-polarized macrophages induce aggressive behavior of human and mouse GC cells.

From: Tumor-associated macrophages-derived exosomes promote the migration of gastric cancer cells by transfer of functional Apolipoprotein E

Fig. 2

a The pie chart shows the mean fraction of immune subpopulations in STAD without (non-metas, n = 115) or with metastasis (metas, n = 26). The column chart shows the fraction of macrophage subpopulations (M0, M1, and M2) in the macrophage. b Quantification of M2/M1 ratio in non-metastatic (n = 115) and metastatic STAD (n = 26), based on TCIA database. c IHC staining and quantification of CD163 expression (M2 marker) in human GC tissues of patients with or without metastasis from our cohort-I (n = 15). d Flow analysis shows the specific markers of murine bone marrow-derived macrophages (BMDMs) differentiated to M1-polarized (M1) or M2-polarized macrophages (M2). Histograms represent two independent experiments with similar results. e Immunofluorescence staining for F4/80 and CD206 in M1 or M2-polarized macrophages from BMDMs. Scale bar represents 25 μm. f Migration and invasion assay of mouse GC cells (MFC) cocultured with M1 or M2 macrophages. Shown is the mean ± s.e.m. of two independent experiments. g Migration and invasion assay of human GC cells (MGC) cocultured with M1 or M2 macrophages. Shown is the mean ± s.e.m. of two independent experiments. h Quantitative analysis of peritoneal metastasis in mice after inoculation with MFC cancer cells educated with M1 or M2-polarized macrophages (n = 10 mice in each group). Error bars represent mean ± s.e.m.; **P < 0.01; ***P < 0.001; n.s. not significant; by one-way analysis of variance (ANOVA) with Tukey’s method for multiple comparisons (b, c, f, g, h)

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