Fig. 4 | Cell Death & Disease

Fig. 4

From: Tumor-associated macrophages-derived exosomes promote the migration of gastric cancer cells by transfer of functional Apolipoprotein E

Fig. 4

Apolipoprotein E is enriched in M2 macrophages-derived exosomes and highly expressed in M2-polarized macrophage. a The protein content of M2-Exos obtained from two isolation methods (Exo-1: ultracentrifugation and Exo-2: ExoQuick™) was determined by mass spectrometry. The top 10 most abundant proteins among a total of 73 common proteins are listed. b, c mRNA and protein expression of ApoE in M1 and M2-polarized macrophages. d Immunofluorescence of ApoE (red) in the M1 and M2 macrophages. Scale bars represent 10 μm. e IHC staining of ApoE for TAMs (blue square) and tumor cells (red square) from human GC tissues. Scale bars represent 50 μm (inset, 10 μm). f Representative IHC staining of CD68 and ApoE in serial sections of human GC tissues. g, h Western blot analysis (g) and quantification of gene expression (h) of ApoE in mouse peritoneal macrophage (PMs) and TAMs isolated from MFC tumor tissue. Shown is the mean ± s.e.m. of two independent experiments. i Quantification of gene expression of APOE in tumor-infiltrating lymphocytes (TILs), cancer-associated fibroblasts (CAFs), and TAMs isolated from human GC tissues. j Western blot analysis of whole-cell lysates of M1 or M2 macrophages and M2-Exos. Error bars represent mean ± s.e.m. **P < 0.01, ***P < 0.001, ****P < 0.0001; n.s. not significant; by one-way ANOVA with Dunnett’s multiple-comparison test (i) or Student’s t-test (b, h)

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