Fig. 5 | Cell Death & Disease

Fig. 5

From: Tumor-associated macrophages-derived exosomes promote the migration of gastric cancer cells by transfer of functional Apolipoprotein E

Fig. 5

Exosomal transfer of Apolipoprotein E from M2 macrophages promotes migration of GC cells. a Immunofluorescence staining of ApoE in MFC cells cultured with M2-Exos (green) or vehicle. Scale bars represent 15 μm. b Western blot analysis of ApoE in MFC and MGC cells cultured with M2-Exos or vehicle control. c Real-time PCR analysis of ApoE mRNA in MFC cells cultured with M2-Exos or vehicle control. d Migration and invasion assay of MFC cells cultured with M2-Exos from Apoe−/− or WT mice. Shown is the mean ± s.e.m. of three independent experiments. e Representative lung tissue images and quantification of lung metastasis in mice administered MFC cells pretreated with exosomes from Apoe−/− or WT BMDMs (n = 5). Error bars represent mean ± s.e.m. **P < 0.01, ***P < 0.001, ****P < 0.0001; n.s., not significant; by one-way ANOVA with Dunnett’s multiple-comparison test (d, e) or Student’s t-test (c)

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