Fig. 6: MiR-302d-3p regulates tube formation of HUVECs through p21Waf1/Cip1.
From: c-Jun-mediated microRNA-302d-3p induces RPE dedifferentiation by targeting p21Waf1/Cip1
a, b Secreted VEGF-A protein were increased in ARPE-19 cells transfected with miR-302d-3p mimic compared to NC mimic (a), and were decreased in ARPE-19 cells transfected with miR-302d-3p inhibitor when compared to NC inhibitor (b). c, d VEGFA mRNA were increased in ARPE-19 cells transfected with miR-302d-3p mimic when compared to cells transfected with NC mimic (c), and were decreased in ARPE-19 cells transfected with miR-302d-3p inhibitor compared to NC inhibitor (d). e–g Tube formation was promoted in HUVECs transfected with miR-302d-3p mimic compared to NC mimic (e, f), and was inhibited in cells transfected with miR-302d-3p inhibitor compared to NC inhibitor (e, g). h–j Tube formation was suppressed in HUVECs transfected with miR-302d-3p mimic plus AcFlag-CDKN1A compared to miR-302d-3p mimic plus empty vector (h, i), and was induced in cells transfected with miR-302d-3p inhibitor plus CDKN1A-siRNA compared to miR-302d-3p inhibitor plus scramble siRNA (h, j). k–m Tube formation was decreased in HUVECs transfected with AcFlag-CDKN1A compared with empty vector (k, l), and was increased in cells transfected with CDKN1A-siRNA compared to scramble siRNA (k, m). *p < 0.05; **p < 0.01; ***p < 0.001. Scale bar = 100 μm
