Fig. 5: SRCIN1 inhibits angiogenesis and SRC/VEGF signaling. | Cell Death & Disease

Fig. 5: SRCIN1 inhibits angiogenesis and SRC/VEGF signaling.

From: MicroRNA-181a promotes angiogenesis in colorectal cancer by targeting SRCIN1 to promote the SRC/VEGF signaling pathway

Fig. 5

a, bWestern blotting analysis of the protein expression levels of SRCIN1, phosphorylated-SRC (Tyr416), phosphorylated-SRC (Tyr527), c-SRC, and VEGF in HT29 cells transfected with control plasmid, SRCIN1 overexpression plasmid, control siRNA, or SRCIN1 siRNA; a representative images; b quantitative analysis. c ELISA analysis of VEGF secretion from HT29 cells after transfection as described in a. d, e TCM derived from HT29 cells transfected with control plasmid, SRCIN1 overexpression plasmid, control siRNA, or SRCIN1 siRNA were added to HUVECs at a final concentration of 75% TCM, scale bars: 100 μm; d representative images of tube formation; e HUVEC branch number. Data are shown as the mean ± SD of three replicates. *P < 0.05; **P < 0.01; ***P < 0.001

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