Fig. 3: Anti-apoptotic effects of AEG-1 transduction in DA neurons on 6-OHDA neurotoxicity.
From: Upregulation of neuronal astrocyte elevated gene-1 protects nigral dopaminergic neurons in vivo
a Western blot analyses show a significant increase in the levels of caspase-3, c-caspase-3, and c-PARP1 in the postmortem tissues of patients with PD compared with CON. &p = 0.014 for caspase-3, &&p = 0.019 for c-caspase-3, and &&&p = 0.009 for c-PARP-1, vs. CON (t-test; n = 4 for each group). b Experimental schematic for Fig. 3c, d. c Representative double immunofluorescence labeling for TH (red) and c-caspase-3 (green) or TH and c-PARP-1 (green) in the mouse SN. AEG-1 upregulation induces reductions in the levels of expression of both c-caspase-3 and c-PARP-1 in TH-positive DA neurons in the SN with 6-OHDA neurotoxicity. Scale bar, 20 μm. d Western blot analyses of the levels of c-caspase-3 and c-PARP-1 in the SN 2 days after 6-OHDA treatment. *p = 0.029, **p = 0.025, #p = 0.032, and ##p = 0.016 vs. CON; ***p = 0.009 and ###p = 0.002 vs. 6-OHDA alone (one-way ANOVA with Tukey’s post hoc test; n = 4 for each group)
