Fig. 5: PGC1α/NRF1 complexes directly bind to and activate PDHA1 and MPC1 promoters. | Cell Death & Disease

Fig. 5: PGC1α/NRF1 complexes directly bind to and activate PDHA1 and MPC1 promoters.

From: PGC1α promotes cholangiocarcinoma metastasis by upregulating PDHA1 and MPC1 expression to reverse the Warburg effect

Fig. 5

a Results of the co-IP assays shows that PGC1α forms a complex with NRF1 in HuCCT1- PGC1α cells. b NRF1 sites from PDHA1 and MPC1 are compared with the consensus. c EMSA were performed with nuclear extracts and radiolabeled probes encompassing the candidate NRF1-binding sequence on the PDHA1 or MPC1 promoter. d The effect of PGC1α and NRF1 on transcriptional activity of PDHA1 and MPC1 promoters, measured by luciferase reporter assays. Data are the mean ± SD of three independent experiments performed in triplicate. e ChIP assay showing the binding of NRF1 to PDHA1 or MPC1 promoter in vivo. The promoter regions of PDHA1 or MPC1 were amplified using the input and immunoprecipitated DNA as templates. ***P < 0.001

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