Fig. 4: H19 promoted SE-induced hippocampal cell apoptosis in vivo. | Cell Death & Disease

Fig. 4: H19 promoted SE-induced hippocampal cell apoptosis in vivo.

From: Long non-coding RNA H19 contributes to apoptosis of hippocampal neurons by inhibiting let-7b in a rat model of temporal lobe epilepsy

Fig. 4

a, b The protein levels of c-Casp3, Bcl-2 and p53 in a hippocampus from KA-induced epileptic rats at 7 days after surgery (n = 3), as determined by western blot. bRepresentative photomicrographs of c-Casp3 and NeuN immunofluorescence in hippocampus from KA-induced epileptic rats at 7 days after KA injection (scale bars = 50 μm). c, d Top, the timeline showing the experimental design. Bottom, quantification of c-Casp3, Bcl-2, Bax, and p53 proteins in CA3 subfield of hippocampus from c H19 overexpression or d H19 knockdown rats with or without KA treatment for 7 days (n = 3–4). The blots were densitometrically quantified, and the data were normalized to GAPDH blot. All data are shown as mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001 versus control (Con) or sham groups (sham, NC+Veh, or Scr+Veh). #p < 0.05, ##p < 0.01, ###p < 0.001 versus NC+KA or Scr+KA group

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