Fig. 4: ZBTB20 deficiency impairs EGFR expression and activation in liver regeneration.

a Hgfr and Egfr mRNA expression levels were in the mutant liver at the indicated time points after PH. qRT-PCR was performed using 36B4 as internal control. The data are presented as box-and-whisker plots. *P < 0.05 versus control (Mann–Whitney test); n = 4–8 at each time point. b EGFR protein expression and activation were attenuated in regenerating liver from mutant mice. Western blot was performed on the whole liver lysate with GAPDH as loading control. The activation of EGFR, AKT, and NF-kB was impaired in regenerating liver at the indicated times after PH in the absence of ZBTB20