Fig. 6: EGFR overexpression restores the impaired hepatocyte proliferation in regenerating liver from Zbtb20-deficient mice. | Cell Death & Disease

Fig. 6: EGFR overexpression restores the impaired hepatocyte proliferation in regenerating liver from Zbtb20-deficient mice.

From: ZBTB20 regulates EGFR expression and hepatocyte proliferation in mouse liver regeneration

Fig. 6

LZB20KO and control mice were i.v. injected with adenoviruses Ad-GFP or Ad-EGFR (0.2 O.D. per mouse) at 48 h prior to PH, and their liver regeneration was analyzed at 48 h after surgeries. BrdU was i.p. injected 2 h before animal sacrifice. a EGFR expression in the liver was assayed by western blotting. b The frequency of BrdU incorporation and Ki67 expression in hepatocytes 48 h after PH. The data are presented as box-and-whisker plots. *P < 0.05; **P < 0.01 (ANOVA test); n = 4–8. c Representative photographs for the immunostaining of BrdU and Ki67 on liver sections at 48 h after PH. Scale bar (upper), 200 μm; scale bar (lower), 50 μm

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