Fig. 7: ZBTB20 overexpression restores EGFR expression and hepatocyte proliferation in regenerating liver from Zbtb20-deficient mice.

LZB20KO and control mice (3–5 months old) were i.v. injected with adenoviruses Ad-GFP or Ad-ZBTB20 (0.1 O.D. per mouse) 14 days prior to PH, and their liver regeneration was analyzed at 48 h after surgeries. BrdU was i.p. injected 2 h before animal sacrifice. a EGFR expression in the liver was assayed by western blotting, and quantified in Supplementary Fig. 11. b The frequency of BrdU incorporation and Ki67 expression in hepatocytes at 48 h after PH. The data are presented as box-and-whisker plots. *P < 0.05; **P < 0.01; n = 4–6. c Representative photographs for the immunostaining of BrdU and Ki67 on liver sections at 48 h after PH. Scale bar (upper), 200 μm; scale bar (lower), 50 μm