Fig. 1: Regulation of surface FcεRI levels by monomeric IgE.
From: IL-3 but not monomeric IgE regulates FcεRI levels and cell survival in primary human basophils
Kinetics of FcεRI a and b and IgE levels c and d on isolated primary human basophils from low ( ≤ 32 kU/L; gray lines) and high ( > 32 kU/L; black lines) serum IgE donors over a 7-day time period. Cells were incubated either in medium alone, with exogenous recombinant human IgE (100 nM), with the disruptive anti-IgE DARPin® protein bi53_79 (5 µM) or with the disruptive anti-IgE DARPin® protein bi53_79 and subsequently after washing with exogenous recombinant human Sus11-IgE. FcεRI and IgE levels were measured by flow cytometry and displayed as geometric mean fluorescence intensity. Statistical significance was calculated by Student’s t-test at day 7 and data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, n = 3
