Fig. 1: E2F7 localises to the nucleolus.
From: Functional interplay between E2F7 and ribosomal rRNA gene transcription regulates protein synthesis
a U2OS cells expressing HA, Flag or GFP-tagged E2F7. HA-E2F7 and Flag-E2F7 were detected with HA and Flag antibodies, respectively. DAPI staining was used to visualise nuclei. b Endogenous E2F7 was detected in asynchronously growing U2OS cells using rabbit anti-E2F7 antibody H300 (Santa Cruz) and rabbit anti-E2F7 antibody PT (Proteintech). c U2OS cells expressing HA wild-type E2F7 (HA-WT7), GFP-E2F7 or DNA-binding domain mutant R185A were treated with 1 nM actinomycin D overnight. HA-E2F7 was detected with anti-HA antibody. d Endogenous E2F7 (rabbit anti-E2F7 PT) and nucleolin were detected in U2OS cells treated with 1 nM actinomycin D. e U2OS cells were transfected HA-E2F7 constructs containing wild-type E2F7 (HA-WT7) or the DNA-binding domain mutants R185A, R334A and the double-mutant R185A/R334A. Cells were treated with 1 nM actinomycin D overnight before fixation and processing for immunofluorescence. Ectopic E2F7 was detected with anti-HA antibody. Scale bars = 10 μm. Arrows indicate E2F7 perinucleolar localisation
