Fig. 2: E2F7 inhibits Pol I activity.
From: Functional interplay between E2F7 and ribosomal rRNA gene transcription regulates protein synthesis
a i U2OS cells were treated with control (GFP) or E2F7 siRNA for 72 h before RNA isolation and RT-qPCR. 47S transcript levels were quantified using two different primer sets (A and B) and results are expressed as fold over control after normalising for GAPDH levels. Graph represents mean ± s.e.m., n = 3 independent experiments, *p < 0.05, Student’s t test. ii Blots represent the level of E2F7 and tubulin. b Nascent nucleolar RNA synthesis was detected after growing asynchronous U2OS cells transfected with HA wild-type E2F7 (WT) or the DNA-binding mutant R185A (DBD) in the presence of 2 mM 5-FUrd for 30 min before fixation and processing for immunofluorescence. RNA incorporated 5-FUrd was detected using anti-BrdU antibody BU-33 and E2F7 with anti-HA. Arrows highlight regions where there is a lack of FUrd incorporation (WT) or regions where FUrd incorporation is unaffected (DBD)
