Fig. 3: E2F7 localises to rRNA promoter.
From: Functional interplay between E2F7 and ribosomal rRNA gene transcription regulates protein synthesis
a U2OS cells were transfected with Flag-E2F7 or vector control. ChIP was performed cells with (+) and without (−) actinomycin D (ActD) treatment (20 nM, 4 h). PCR was performed using primers that amplify rDNA promoter −144 to +20. b An endogenous ChIP was performed in U2OS cells with (+) and without (−) actinomycin D (ActD) treatment (20 nM, 4 h). PCR was performed using primers that amplify rDNA promoter (i) −144 to +20, and the E2F1 promoter (ii). c ChIP was performed in U2OS cells treated for 72 h with either GFP siRNA or E2F7 siRNA using rabbit anti-UBF or rabbit IgG. i Schematic represents the Pol I promoter and the approximate positions of the primer sets used are shown. ii Graph represents range ±SD. qPCR was performed on ChIP chromatin using actin as a control gene. Results are expressed as fold over IgG (rabbit non-specific IgG) after normalising to input levels. n = 3 independent experiments. iii Blots on extracts demonstrating E2F7 depletion. E2F7 was detected using rabbit anti-E2F7 antibody and actin was used as a loading control
