Fig. 4: The capacity of hsa-miR-12528 during tumourigenesis in an in vivo model. | Cell Death & Disease

Fig. 4: The capacity of hsa-miR-12528 during tumourigenesis in an in vivo model.

From: The novel hsa-miR-12528 regulates tumourigenesis and metastasis through hypo-phosphorylation of AKT cascade by targeting IGF-1R in human lung cancer

Fig. 4

Influence of miR-12528 during tumourigenesis in vivo. A549 cells were subcutaneously injected in equal amounts into both flanks in an individual mouse. After stabilisation for 5 weeks, the left tumours were only injected with PBS, whereas the right tumours were injected with either NC, miR-12528 or ASO-12528 mimics twice weekly. After a total of 4 weeks from the time of mimic injection, 7 mice per group were sacrificed (a total of 21 mice) and the excised tumour mass was imaged and measured (a). The rations of tumour volumes indicate that the right tumours injected with NC, miR-12528 or ASO-12528 mimics were normalised to a left tumour (MOCK) within each individual mouse (b). The tumour sections were expressed with H&E staining, Ki67-IHC and TUNEL assay. The H&E staining result shows the histological morphology of tumour tissues. Positive results for Ki67 and TUNEL, markers of proliferation and apoptosis, were indicated by brown nuclear staining (c)

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