Fig. 2: Tunicamycin induces appearance of a non-N-glycosylated form of mTRAIL-R.

a MEFs were treated with CTRL, TU, or THAP for 17 h, and cell lysates were immunoblotted for mTRAIL-R and β-tubulin. Asterisk indicates non-specific signal. Representative images of three independent experiments. b MEFs were transfected with non-silencing (NS) or mTRAIL-R specific siRNA, and then treated with TU for 17 h. Cell lysates were immunblotted for mTRAIL-R and β-tubulin. Asterisk indicates non-specific signal. Representative images of three independent experiments. c Cell lysates of CTRL- or TU-treated MEFs were incubated at 37 °C for 1 h in presence or absence of PNGase F (PNG), and then immunoblotted for mTRAIL-R and β-tubulin. Asterisk indicates non-specific signal. Representative images of two independent experiments. d MEF cells were treated with TU for increasing times and cell lysates were immunblotted for mTRAIL-R and β-tubulin. Asterisk indicates non-specific signal. Representative images of three independent experiments