Fig. 7: YAP/TAZ mediates ACTL6A-regulated proliferation, migration and invasion in glioma cells. | Cell Death & Disease

Fig. 7: YAP/TAZ mediates ACTL6A-regulated proliferation, migration and invasion in glioma cells.

From: Actin like-6A promotes glioma progression through stabilization of transcriptional regulators YAP/TAZ

Fig. 7

a Western blot analysis of lysates prepared from ACTL6A-interfered glioma cells with YAP/TAZ knockdown or active forms ectopic expression. GAPDH was used as loading control. b CCK8 assay performed on ACTL6A-interfered glioma cells with YAP/TAZ knockdown or active forms ectopic expression. Data are represented as the mean ± SEM. c Colony forming assay performed on ACTL6A-interfered glioma cells with YAP/TAZ knockdown or active forms ectopic expression. Graphic representation of the colony numbers under each condition. Data are represented as the mean ± SEM. d Graphic representation of migrated and invaded cells counts from transwell assays performed in ACTL6A-interfered glioma cells with YAP/TAZ knockdown or active forms ectopic expression. Data are represented as the mean ± SEM from three independent experiments. Scale bars, 100 µm. e Representative images of subcutaneous xenografts from ACTL6A-interfered glioma cells with YAP/TAZ knockdown or active forms ectopic expression after surgical remove. f Tumor growth curves from indicated groups. g Tumor weight in nude mice from indicated groups. Student’s t-test: *P < 0.05, **P < 0.01, ***P < 0.001

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