Fig. 1: KLC4 protein as a radioresistance and tumorigenesis biomarker. | Cell Death & Disease

Fig. 1: KLC4 protein as a radioresistance and tumorigenesis biomarker.

From: Kinesin light chain-4 depletion induces apoptosis of radioresistant cancer cells by mitochondrial dysfunction via calcium ion influx

Fig. 1

a Lung cancer cell lines were treated with 10 Gy radiation for 48 h. Cell death was determined by FACS analysis. b In lung cancer cell lines, protein levels of KLC and KHC family members were determined by Western blotting (left) and qRT-PCR (right). c Representative microscopic images of lung cancer tissues and their normal tissue counterparts stained with KLC4 antibody (scale bar: 50 μm). Staining intensity was scored as follows: 0, no staining; +1, weak; +2, moderate; and +3, strong. Data are presented as box-and whisker plots. d In lung cancer, localization of KLC4 has been visualized by confocal microscopy, and representative images are shown; magnification ×400. All data are shown as the mean ± S.D. of at least three independent experiments. The P-values were calculated using unpaired Student’s t-test. *P < 0.05; **P < 0.005; ***P < 0.001

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