Fig. 2: KLC4 depletion with radiation treatment induces apoptosis and anti-proliferative effects in lung cancer cells. | Cell Death & Disease

Fig. 2: KLC4 depletion with radiation treatment induces apoptosis and anti-proliferative effects in lung cancer cells.

From: Kinesin light chain-4 depletion induces apoptosis of radioresistant cancer cells by mitochondrial dysfunction via calcium ion influx

Fig. 2

a Expression of the KLC4 protein by Western blot analysis following transfection with 40 nM control siRNA (siCon) or three different KLC4 siRNAs (siKLC4s) in R-H460 and A549 cells. b R-H460 was treated with 10 Gy radiation for 48 h after siKLC4 transfection. Protein levels of cleaved PARP and cleaved caspase3 determined by Western blotting and c cell death in R-H460 cells treated as in b determined by Annexin V/PI staining, performed as described in the Materials and Methods. d, e Surviving fraction of R-H460 and A549 cells treated with a single dose of radiation (0–8 Gy) after transfection with 40 nM control siRNA or KLC4 siRNA, measured 2 weeks after radiation treatment. All data are shown as the mean ± S.D. of at least three independent experiments. The P-values were calculated using unpaired Student’s t-test. *P < 0.05; **P < 0.005; ***P < 0.001

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