Fig. 3: Loss of KLC4 promotes radiosensitivity in vivo.
a The effect of KLC4 knockdown with irradiation on tumorigenicity by subcutaneous injection of 1 × 106 R-H460 cells. After 7 days, the mice were subjected to tail vein injection every 2 days with control siRNA (siCon) or KLC4 siRNA (siKLC4) (40 μg siRNA/mouse). Ten days after the injection of R-H460 cells, 6 Gy X-ray radiation was delivered to R-H460 xenograft tumors. Tumor volume was calculated at the indicated times using the following formula: Volume = (Length × Width2 × 3.14)/6 (n = 6). b Tumors were excised and weighed at the end of the experiment (28 days). Representative images of tumors and a graph of tumor weight. All data are shown as the mean ± S.D. and the P-values were calculated using unpaired Student’s t-test. *P < 0.05; ***P < 0.001. c Immunohistochemical staining (top) and TUNEL staining (bottom) of KLC4 xenograft tumor sections after treatment;magnification ×400. d Western blot analysis was performed to determine protein levels of KLC4 and cleaved PARP in all samples per group in KLC4 xenograft tumors. All data are shown as the mean ± S.D. of at least three independent experiments. The P-values were calculated using unpaired Student’s t-test. *P < 0.05; **P < 0.005; ***P < 0.001
