Fig. 6: Mitochondrial dysfunction through mitochondrial calcium uptake in KLC4-depleted cells. | Cell Death & Disease

Fig. 6: Mitochondrial dysfunction through mitochondrial calcium uptake in KLC4-depleted cells.

From: Kinesin light chain-4 depletion induces apoptosis of radioresistant cancer cells by mitochondrial dysfunction via calcium ion influx

Fig. 6

a KLC4 siRNA-treated cells were stained with Rhod-2 and MitoTracker Green, a mitochondrial marker, and imaged by confocal microscopy. b Rhod-2 fluorescence determination by FACS analysis after KLC4 siRNA transfection and treatment with ruthenium red (RR) to inhibit mitochondrial calcium uptake in R-H460 and A549 cells. c Damaged mitochondria determination by FACS analysis after siKLC4 transfection and RR treatment. d Mitochondrial ROS determination by FACS analysis after siKLC4 transfection and RR treatment. e The KLC4 siRNA and RR-treated R-H460 and A549 cells were stained with Annexin V and propidium iodide (PI) and then analyzed by FACS analysis. f Effects of RR on KLC4 siRNA treated-induced caspase-3 activation and PARP cleavage were analyzed by Western blot analysis. All data are shown as the mean ± S.D. of at least three independent experiments. The P-values were calculated using unpaired Student’s t-test. *P < 0.05; **P < 0.005; ***P < 0.001

Back to article page