Fig. 7: The biological functions of KLC4 in cervical cancer cells. | Cell Death & Disease

Fig. 7: The biological functions of KLC4 in cervical cancer cells.

From: Kinesin light chain-4 depletion induces apoptosis of radioresistant cancer cells by mitochondrial dysfunction via calcium ion influx

Fig. 7

a Cervical cancer cell lines were treated with 10 Gy radiation for 48 h. Cell death was determined by FACS analysis. b In cervical cancer cells, protein levels of KLC and KHC family members were determined by Western blotting. c The available data set in the Oncomine database was queried for KLC4 expression with respect to cervical cancer vs. normal tissues. d Damaged mitochondria determination by FACS analysis after siKLC4 plus RR treatment in SiHa cells. e MitoSox determination by FACS analysis after siKLC4 plus RR treatment in SiHa cells. f The KLC4 siRNA and RR-treated SiHa cells were stained with Annexin V and propidium iodide (PI) and then analyzed by FACS analysis. All data are shown as the mean ± S.D. of at least three independent experiments. The P-values were calculated using unpaired Student’s t-test. *P < 0.05; **P < 0.005; ***P < 0.001

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