Fig. 5: AMPK could directly control MFF/Drp1 pathway in MSCs with iron overload. | Cell Death & Disease

Fig. 5: AMPK could directly control MFF/Drp1 pathway in MSCs with iron overload.

From: Iron overload promotes mitochondrial fragmentation in mesenchymal stromal cells from myelodysplastic syndrome patients through activation of the AMPK/MFF/Drp1 pathway

Fig. 5

a Total protein expression of MFF, p-MFF-ser155, Drp1 and mitochondrial protein expression of p-Drp1-ser616, p-Drp1-ser637 in WT MSCs or control CrispCas MSCs or AMPKα DKO MSCs before and after treatment with FAC or AICAR. b Protein expression of AMPKα, p-AMPKα-thr172 and p-ACC-ser79 in control shRNA MSCs or MFF shRNA MSCs before and after adding FAC or AICAR. c Total protein expression of MFF, p-MFF-ser155, Drp1, LC3, Beclin-1, ATG5 and ATG7, and mitochondrial protein expression of p-Drp1-ser616, p-Drp1-ser637 in control shRNA MSCs or MFF shRNA MSCs before and after adding AICAR. The average of three replicates is displayed

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