Fig. 1: C2EIP encodes a cytoplasmic protein that is differentially expressed in PGC.

a Transcriptome analysis of ESC, PGC, and SSC. The differential expression of genes in these cells can be divided into six clusters.Cluster1 represents genes with high expression in ESC but low expression in PGC and SSC; Cluster2 represents genes with high expression in ESC and PGC but low expression in SSC; Cluster3 represents genes with high expression in PGC but low expression in ESC and SSC; Cluster4 represents genes with high expression in PGC and SSC but low expression in ESC; Cluster5 represents genes with high expression in SSC but low expression in ESC and PGC; Cluster6 represents genes with high expression in ESC and SSC but low expression in PGC. b The distribution of differentially expressed genes in ESC vs. PGC and PGC vs. SSC indicate C2EIP is highly expressed in PGC. Red represents upregulated genes, green represents down-regulated genes. c The RPKM value of C2EIP, representing relative expression, in ESC, PGC, and SSC. d qRT-PCR analysis of C2EIP expression in indicated tissues, ESC, PGC, SSC, and RA-induced cells. e Western blot analysis of C2EIP protein levelsESC, PGC, and SSC.β-actin was used as an internal reference. f Confirmation of C2EIP-EGFP fusion protein expression. Cells were transfected with EGFP-N1 vector as a positive control, or mock-transfected with double distilled water (DDW) as a negative control. g Indirect immunofluorescence showing cytoplasmic localization of C2EIP. Cells transfected with pcDNA3.0 vector were used as a negative control, Scale bar:20μm