Fig. 2: High C2EIP expression in PGC is mediated by promoter histone modification. | Cell Death & Disease

Fig. 2: High C2EIP expression in PGC is mediated by promoter histone modification.

From: Interaction of the primordial germ cell-specific protein C2EIP with PTCH2 directs differentiation of embryonic stem cells via HH signaling activation

Fig. 2

a Schematic diagram of C2EIP promoter fragment cloning and vector construction. The double luciferase reporter system was used to examine the effects of 5-Zacd (b) and TSA (c) on C2EIP promoter activity. d CHIP-qPCR was used to detect histone acetylation in the C2EIP promoter region. e Luciferase activity in DF-1 co-transfected with PGL3.0/787, PGL3.0/588, PGL3.0/347,or PGL3.0/170. DF-1 cells transfected with PGL3.0/basic was a negative control. f Top:the location of STAT1, STAT10, Sox17, Sox2, and Klf5 in the core region of the C2EIP promoter; Bottom:diagram of STAT1 transcription factor binding site mutation. g The double luciferase reporter system was used to assess C2EIP promoter activity when the STAT1 transcription factor binding site was mutated (Mut) or STAT1 was overexpressed from the pcDNA3.0-STAT1 plasmid

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