Fig. 4: Identification of PDK2 as an miR-422a target in GC. | Cell Death & Disease

Fig. 4: Identification of PDK2 as an miR-422a target in GC.

From: MiR-422a regulates cellular metabolism and malignancy by targeting pyruvate dehydrogenase kinase 2 in gastric cancer

Fig. 4

a qRT-PCR analysis of five candidate genes after miR-422a overexpression in SGC7901 cells. b miR-422a targeting sites in the 3′-UTR of targets genes and corresponding mutations. c A luciferase reporter assay was used to evaluate interactions between miR-422a and the targeting sites of candidate genes in SGC7901 cells. d Levels of PDK2, total PDH and phosphorylated PDH-E1α protein in cancer cells with miR-422a overexpression or knockdown. e After miR-422a overexpression in SGC7901 and miR-422a knockdown in MGC803 cells, the cells were harvested for measurement of cellular PDH activity. f Overall survival analysis of the patients based on PDK2 and PDH expression in the KM-plotter database. The error bars represent the mean (n = 3) ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001 versus corresponding NC

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