Fig. 6: Analysis of the effects of the miR-422a–PDK2 axis on ROS production and cell cycle progression in GC. | Cell Death & Disease

Fig. 6: Analysis of the effects of the miR-422a–PDK2 axis on ROS production and cell cycle progression in GC.

From: MiR-422a regulates cellular metabolism and malignancy by targeting pyruvate dehydrogenase kinase 2 in gastric cancer

Fig. 6

a FACS analysis (above) and statistical results (below) of ROS levels after miR-422a overexpression or PDK2 knockdown in SGC7901 cells. b FACS analysis (above) and statistical results (below) of ROS levels after miR-422a knockdown or PDK2 overexpression in MGC803 cells. c DNA content analysis of SGC7901 cells after transfection with Lv-miR-422a, sh-PDK2, negative control, or 10 mM NAC for 24 h as indicated. d DNA content analysis of MGC803 cells after transfection with anti-miR-422a, PDK2, negative control, 20 μM or 150 μM H2O2 for 24 h. The error bars represent the mean (n = 3) ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001 versus corresponding NC

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