Fig. 4: Overexpression of Src reverts the effect of OC and enhances the chemoresistant of pancreatic cancer cells.

a MIA-RES and MIA PaCa-2 cells were transfected with 2 μg Src plasmid or empty vector (Vehicle) for 24 h. The expression of Src mRNA (left) and protein (right) were detected by qPCR and western blot analysis. TBP served as an endogenous control. β-actin was used as a loading control. Cell viability levels of MIA-RES b and MIA PaCa-2 c were measured by CCK8 assay in Src overexpression (Gray bars) and empty vector (Black bars). Treatments are indicated at the bottom of the graph for 48 h. Data represent the average of three independent experiments ± standard deviation. *p < 0.05 and **p < 0.01 ***p < 0.001. Relative Src mRNA expression levels of MIA-RES d and MIA PaCa-2 cells e were determined after transfected with empty or Src plasmid, followed by the treatment with or without 10 μM OC for 24 h. f MIA-RES and MIA PaCa-2 cells g were transfected with Src expression vector or empty vector for 24 h, followed by DMSO or OC treatment for 24 h. Cell lysates were analyzed by immunoblotting. β-actin expression was used as a loading control.