Fig. 5: OC promotes Src protein degradation by the ubiquitin-proteasome pathway. | Cell Death & Disease

Fig. 5: OC promotes Src protein degradation by the ubiquitin-proteasome pathway.

From: Natural compound Oblongifolin C confers gemcitabine resistance in pancreatic cancer by downregulating Src/MAPK/ERK pathways

Fig. 5

MIA-RES cells were treated with OC for the indicated dose (0–10 μM) a and indicated time periods (0–48 h) b, then the level of ubiquitination was detected by western blotting using an anti-ubiquitin antibody. c MIA-RES cells were treated with 50 μM cycloheximide (CHX) with or without 10 μM OC for the indicated time and the cell lysates were analyzed by immunoblotting. β-actin served as a loading control. d MIA-RES cells were co-treated with 15 μM MG132 with or without 10 μM OC and harvested at 0, 3, 6,12, and 24 h after treatment for western blotting analysis. β-actin served as a loading control. e MIA-RES cells were transfected with 2 μg Src plasmid for 24 h, and treated with 10 μM OC for 24 h. The cells were treated with 15 μM MG132 for 6 h before collected, and 6xHis-tagged proteins of Src were purified with Ni-NTA Agarose beads, followed by western blotting analyses to detect the level of Src ubiquitination.

Back to article page