Fig. 6: OC suppresses orthotopic pancreatic tumor growth and improves the chemosensitivity of GEM in vivo.
MIA PaCa-2 cells (1 × 106 cells per mouse) were implanted directly into the pancreas of nude mice. One week later, the mice received intraperitoneal injections of either vehicle control (0.05% DMSO in normal saline), 20 mg/kg OC every 2 days, or GEM 20 mg/kg twice a week. Body weights a of the animals were measured every other day. After four weeks, the mice were killed, tumor weight b, and tumor volume c were measured. *p < 0.05, **p < 0.01, ***p < 0.001 versus the corresponding control groups (n = 6). d Paraffin-embedded orthotopic tumor tissues sections were stained with HE or Ki-67, cleaved caspase-3 antibodies (Scale bar: 20 μm). For the GEM-resistant cellular orthotopic implantation, 1 × 106 MIA-RES cells were injected into the pancreas of each mice. One week later, the mice received intraperitoneal injections of either vehicle control (0.05% DMSO in normal saline), OC (10 mg/kg) every day and GEM (20 mg/kg) twice a week alone or in combination with OC. Body weights e of the animals were measured every day. After 2 weeks of treatment, the mice were killed, tumor weight f, and tumor volume g were measured. h Paraffin-embedded orthotopic tumor tissues sections were stained with HE, Ki-67, Src and p-Src (Tyr416), cleaved caspase-3 antibodies (Scale bar: 20 μm).
