Fig. 2: Downregulation of DDB2 promotes the conversion of ALDH⁻ to ALDH⁺ cells in ovarian cancer cells.

a Sorting of ALDH⁻ cells (purple) from 2008-pTRIPZ-shDDB2-c1 cells. b–d ALDH⁻ cells were cultured in the absence or presence of Dox for 12 days, DDB2 expression was determined using immunoblotting (b), the percentage of ALDH⁺ cells (blue) was determined using FACS (c, d). N = 3, Bar: SD, **P < 0.01. e Sorting of ALDH⁻ cells (purple) from OVCAR4 cells. f–h ALDH⁻ cells were cultured for 12 days, and transfected with control or DDB2 siRNA every 3 days during this period. DDB2 expression was determined using immunoblotting (f), the percentage of ALDH⁺ cells (blue) was determined using FACS (g, h). N = 3, Bar: SD, **P < 0.01. Note: DEAB was used as a negative control for gating of ALDH⁺ cells