Fig. 3: Strengthening EMC potentiates neuronal cell death.
From: SK channel activation is neuroprotective in conditions of enhanced ER–mitochondrial coupling
a MTT Assay following glutamate treatment (8 mM) for 12–18 h. Data are presented as mean ± SD, n = 8, Student's t-test ***p < 0.0001 compared to untreated control. b MTT assay following treatment with glutamate (8 mM, 16 h) in the presence or absence of rapamycin (0, 50, 100, 150, 200 nM). Data are presented as mean ± SD, n = 6, Student’s t-test ***p < 0.0001 compared to untreated control. c xCELLigence measurement of HT22 cells transfected with ER-FRB-CFP and TOM70-FKBP12-RFP (EML) following addition of glutamate (8 mM, 16 h) in the presence or absence of rapamycin (100 nM). Data are presented as mean ± SD, n = 6. d MTT Assay in HT22 cells transfected with TOM70-FKBP12-RFP and either ER-CFP (ER, gray bars) or ER-FRB-CFP (EML, blue bars) following treatment with glutamate (4 mM, 16 h) and rapamycin (100 nM). Data are presented as mean ± SD, n = 6, Student’s t-test ***p < 0.0001 compared to glutamate in ER control
