Fig. 1: cZRANB1 expression is significantly upregulated during glaucoma-induced retinal neurodegeneration.

a The amplified product of cZRANB1 using the total RNAs of rat retinal lysates was sent for Sanger sequencing, and then compared with mouse cZRANB1 sequence as  recorded in circBase. b Total RNAs from rat retinas were digested with RNase R followed by qRT-PCRs detection of cZRANB1 expression. ZRANB1 mRNA was detected as the RNase R-sensitive control (n = 4). c, d Glaucoma was induced by anterior chamber injection of microbeads. IOP levels in the eyes injected with PBS buffer (sham eyes) and microbeads (injected eyes) were shown (c, n = 5). qRT-PCRs were conducted to detect cZRANB1 expression in the retinas of rats after microbead injection at the indicated time points (d, n = 5). e The aqueous humor was obtained fromglaucoma patients (n = 25) and cataract patients (n = 25) without ocular neurodegenerative diseases. qRT-PCRs were conducted to detect cZRANB1 expression. All data were from at least three independent experiments