Fig. 1: Identification and quantitation of endogenous authentic OCT4A transcripts in somatic cancer cells.
From: Endogenous authentic OCT4A proteins directly regulate FOS/AP-1 transcription in somatic cancer cells
a The target locations of OCT4A-128 and OCT4A-1184 primers relative to the POU5F1 gene. b Determination of the efficiency of gDNA elimination mediated by gDNA eraser in the PrimeScript RT reagent kit. Total RNAs of HeLa cells treated with or without gDNA eraser and HeLa cDNAs reverse transcribed from total RNAs treated with gDNA eraser were subjected to PCR analysis. NCCIT cDNAs were used as a positive control and no template (H2O) as a negative control. c Detection of a small fragment (OCT4A-128) and the full-length (OCT4A-1184) transcript of OCT4A in multiple somatic cancer or normal cell lines by RT-PCR. For NCCIT, the amounts of PCR products of OCT4A-128 and OCT4A-1184 loaded were only 1/70 of other cells. d qRT-PCR analysis of the cell lines in (c), with OCT4A-total, OCT4A-128, and OCT4A-158 primers, GAPDH was used as an internal control. e FPKM of transcripts of POU5F1 and POU5F1 pseudogenes in HeLa and NCCIT cells
