Fig. 4: RXRα downregulated angiogenesis-related makers partially through interacting with ERα. | Cell Death & Disease

Fig. 4: RXRα downregulated angiogenesis-related makers partially through interacting with ERα.

From: RXRα provokes tumor suppression through p53/p21/p16 and PI3K-AKT signaling pathways during stem cell differentiation and in cancer cells

Fig. 4

a The expression of EPHB4, KDR, and eNOs in HUVECs overexpressing RXRα. b The expression of ERα in cancer cells MCF-7 and HeLa, endothelial cells HUVECs, HMVECs, and HAVECs. c Co-IP analysis of protein–protein interaction between RXRα and ERα (experiment was performed as described in “Materials and methods”). d Knockdown of endogenous RXRα in HUVECs with siRXRα. e The expression of ERα examined by qRT-PCR and western blotting in HUVECs following siRXRα knockdown. f Immunocytochemistry analysis of ERα in HUVECs. g Binding of RXRα on the promoter of ERα determined by luciferase assay. A plasmid containing the ERα promoter was transfected into COS-7 cells together with plasmids expressing ERa or/and RXRα, and luciferase activities were measured 24 h later. h The expression of EPHB4, KDR, and eNOs in HUVECs with siRXRα knockdown or/and ERα overexpression. For the measurements of luciferase reporter and mRNA level, the means ± SD from three independent experiments are shown (*P < 0.05; **P < 0.01, n = 3)

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