Fig. 1: Autophagy induction by S. Typhimurium. | Cell Death & Disease

Fig. 1: Autophagy induction by S. Typhimurium.

From: Activation of TGF-β-activated kinase 1 (TAK1) restricts Salmonella Typhimurium growth by inducing AMPK activation and autophagy

Fig. 1

HeLa cells were infected with indicated MOI of S. Typhimurium for 2 h (a) or with 10 MOI of S. Typhimurium for the indicated lengths of time (b). Cell lysates were prepared and analyzed for LC3-II lipidation, p62 and actin by western blot with the indicated antibodies. c, d Increased LC3-II lipidation was not due to the stall of autophagy flux. HeLa cells pretreated with bafilomycin (Baf; 5 or 20 nM) or chloroquine (CQ; 5 or 20 μM) for 30 min were infected with S. Typhimurium (10 MOI) for 2 h. Cell lysates were prepared and analyzed for LC3-II lipidation, p62, and actin by western blot with the indicated antibodies. Blots for LC3-II lipidation, p62, and actin were semi-quantified using NIH Image-J software and presented as bar graphs. Data are presented as the mean ± SD (n = 3) relative to control. *p < 0.05, **p < 0.01, compared to uninfected control; #p < 0.05, ##p < 0.01, compared to S. Typhimurium infection

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