Fig. 2: Time course of AGEs on expression of AGER, ROS generation, and apoptosis in ADSCs. | Cell Death & Disease

Fig. 2: Time course of AGEs on expression of AGER, ROS generation, and apoptosis in ADSCs.

From: miR-5591-5p regulates the effect of ADSCs in repairing diabetic wound via targeting AGEs/AGER/JNK signaling axis

Fig. 2

a Time course of AGER protein expression in ADSCs treated with AGEs (800 μg/ml). b qPCR analysis of mRNA levels of AGER after cells treated with AGEs at different time. c Intracellular ROS generation was visualized under the fluorescence microscope. d Flow cytometer was employed to detect ROS level. e ADSCs were stained with annexin V-FITC/PI and immediately analyzed by flow cytometry. f Western blot analysis of protein levels of caspase-3 and PARP. Every experiment repeated at least three times. Error bars indicate mean ± SD (*P < 0.05)

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