Fig. 1: Irradiated tumor cells release HMGB1. | Cell Death & Disease

Fig. 1: Irradiated tumor cells release HMGB1.

From: HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect

Fig. 1

a Irradiated tumor cells released HMGB1. Upper panel, the western blot results of HMGB1 in cell supernatant; Lower panel, HMGB1 relative densitometry units were calculated. b Cell compartmentalization. HMGB1 is localized in both nucleus and cytoplasm of tumor cells. GAPDH and Lamin B1, loading controls for the cytoplasmic and nuclear fractions, respectively. Lower panel, HMGB1 relative densitometry units were calculated. c Immunofluorescence staining of HMGB1. HMGB1 is detected in both nucleus and cytoplasm of irradiated tumor cells but mainly in nucleus of non-irradiation tumor cells. *p <0.05; **p <0.01

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