Fig. 3: HMGB1 released from irradiated tumor cells promotes cancer cell repopulation via binding to RAGE. | Cell Death & Disease

Fig. 3: HMGB1 released from irradiated tumor cells promotes cancer cell repopulation via binding to RAGE.

From: HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect

Fig. 3

a Analysis the HMGB1 centered interaction subnetwork from STRING database. b Expression changes of RAGE in HT29 and HeLa cell. Left panel, expression changes of RAGE in 10Gy-irradiated HMGB1 WT and KO tumor cells at different time points. Right panel, expression changes of RAGE in HMGB1 WT tumor cells treated by 10Gy-irradiated HMGB1 WT or KO tumor cell culture supernatant collected from indicated time points. c Flow cytometry analysis of surface localized RAGE in HeLa cell. Lower panel, a histogram showed the percentage of RAGE positive cells. d Co-immunoprecipitation analysis of the binding between HMGB1 and RAGE in HeLa cells. e Inhibition of RAGE with FPS-ZM1 significantly recedes the ability of irradiated tumor cells to promote proliferation of Fluc-labeled tumor cells. *p <0.05; **p <0.01

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